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polyclonal primary goat anti cd4 antibody  (R&D Systems)


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    R&D Systems polyclonal primary goat anti cd4 antibody
    Fig. 2. T cell clonality in pulmonary inflammation. (A) Blood-lung activation map of T cells from blood and BALF of all patients: UMAP dimensionality reduction em- bedding of T cells (left); clone size proportion (clone count divided by number of cells per sample) of T cells (middle), and the cytokine secretion score of T cells (right) from COVID-19 and bacterial pneumonia as indicated. (B) UMAP presentation of T cells from BALF of all patients. Clusters were annotated according to gene expression and epitopes measurement of key markers. TCM, T central memory; TSCM, T stem cell–like memory; lncRNA, long noncoding RNA. (C) Ratio of clonal expansion of bacterial pneumonia versus COVID-19 for the major expanded BALF T cell clusters. (D) Subclustering analysis of clonally expanded <t>CD4+</t> T cells of all patients. Clusters were anno- tated according to gene expression presented in the heatmap. (E) Volcano plot showing differential gene expression between TH17 clusters 1 and 2 of all patients. Genes were considered significant with adjust P < 0.05. Nonsignificant genes are shown in black. (F) Heatmap of selected pathogenic gene markers of TH17 cells of all patients in comparison with other T cell clusters. (G) Clone size proportion of T cells in peripheral blood and BALF of patients with COVID-19 and presentation of high abundant clones (clone size > 5) that are shared between BALF and blood and BAL-specific clones as indicated. (H) CD4 migration and tissue residency score of TH17 cluster1 (TRM17) and 2 (TEM17) from all patients. (I) Possible model of intraclonal diversification of CD4+ T cell subsets (left); distribution of two representative BALF clones from a patient with COVID-19 (patient S1 clone239 and clone218) on the UMAP (middle and right). (J) Bar plot of top expanded BALF clones containing TRM17 cells from patients with COVID-19. COVID-19: n = 8 for BALF and n = 7 for blood; bacterial pneumonia: n = 4 for BALF and n = 4 for blood.
    Polyclonal Primary Goat Anti Cd4 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal primary goat anti cd4 antibody/product/R&D Systems
    Average 94 stars, based on 55 article reviews
    polyclonal primary goat anti cd4 antibody - by Bioz Stars, 2026-05
    94/100 stars

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    1) Product Images from "Clonal expansion and activation of tissue-resident memory-like Th17 cells expressing GM-CSF in the lungs of severe COVID-19 patients."

    Article Title: Clonal expansion and activation of tissue-resident memory-like Th17 cells expressing GM-CSF in the lungs of severe COVID-19 patients.

    Journal: Science immunology

    doi: 10.1126/sciimmunol.abf6692

    Fig. 2. T cell clonality in pulmonary inflammation. (A) Blood-lung activation map of T cells from blood and BALF of all patients: UMAP dimensionality reduction em- bedding of T cells (left); clone size proportion (clone count divided by number of cells per sample) of T cells (middle), and the cytokine secretion score of T cells (right) from COVID-19 and bacterial pneumonia as indicated. (B) UMAP presentation of T cells from BALF of all patients. Clusters were annotated according to gene expression and epitopes measurement of key markers. TCM, T central memory; TSCM, T stem cell–like memory; lncRNA, long noncoding RNA. (C) Ratio of clonal expansion of bacterial pneumonia versus COVID-19 for the major expanded BALF T cell clusters. (D) Subclustering analysis of clonally expanded CD4+ T cells of all patients. Clusters were anno- tated according to gene expression presented in the heatmap. (E) Volcano plot showing differential gene expression between TH17 clusters 1 and 2 of all patients. Genes were considered significant with adjust P < 0.05. Nonsignificant genes are shown in black. (F) Heatmap of selected pathogenic gene markers of TH17 cells of all patients in comparison with other T cell clusters. (G) Clone size proportion of T cells in peripheral blood and BALF of patients with COVID-19 and presentation of high abundant clones (clone size > 5) that are shared between BALF and blood and BAL-specific clones as indicated. (H) CD4 migration and tissue residency score of TH17 cluster1 (TRM17) and 2 (TEM17) from all patients. (I) Possible model of intraclonal diversification of CD4+ T cell subsets (left); distribution of two representative BALF clones from a patient with COVID-19 (patient S1 clone239 and clone218) on the UMAP (middle and right). (J) Bar plot of top expanded BALF clones containing TRM17 cells from patients with COVID-19. COVID-19: n = 8 for BALF and n = 7 for blood; bacterial pneumonia: n = 4 for BALF and n = 4 for blood.
    Figure Legend Snippet: Fig. 2. T cell clonality in pulmonary inflammation. (A) Blood-lung activation map of T cells from blood and BALF of all patients: UMAP dimensionality reduction em- bedding of T cells (left); clone size proportion (clone count divided by number of cells per sample) of T cells (middle), and the cytokine secretion score of T cells (right) from COVID-19 and bacterial pneumonia as indicated. (B) UMAP presentation of T cells from BALF of all patients. Clusters were annotated according to gene expression and epitopes measurement of key markers. TCM, T central memory; TSCM, T stem cell–like memory; lncRNA, long noncoding RNA. (C) Ratio of clonal expansion of bacterial pneumonia versus COVID-19 for the major expanded BALF T cell clusters. (D) Subclustering analysis of clonally expanded CD4+ T cells of all patients. Clusters were anno- tated according to gene expression presented in the heatmap. (E) Volcano plot showing differential gene expression between TH17 clusters 1 and 2 of all patients. Genes were considered significant with adjust P < 0.05. Nonsignificant genes are shown in black. (F) Heatmap of selected pathogenic gene markers of TH17 cells of all patients in comparison with other T cell clusters. (G) Clone size proportion of T cells in peripheral blood and BALF of patients with COVID-19 and presentation of high abundant clones (clone size > 5) that are shared between BALF and blood and BAL-specific clones as indicated. (H) CD4 migration and tissue residency score of TH17 cluster1 (TRM17) and 2 (TEM17) from all patients. (I) Possible model of intraclonal diversification of CD4+ T cell subsets (left); distribution of two representative BALF clones from a patient with COVID-19 (patient S1 clone239 and clone218) on the UMAP (middle and right). (J) Bar plot of top expanded BALF clones containing TRM17 cells from patients with COVID-19. COVID-19: n = 8 for BALF and n = 7 for blood; bacterial pneumonia: n = 4 for BALF and n = 4 for blood.

    Techniques Used: Activation Assay, Gene Expression, Comparison, Clone Assay, Migration

    Fig. 5. Cytokine secretion profile and cellular source of GM-CSF. (A) GM-CSF and IL-17A protein in serum of patients with COVD-19 (n = 8) and healthy controls (n = 7) from Hamburg and of patients with moderate (n = 8) or severe COVID-19 (n = 11) from Halle as indicated. Cell map of (B) CSF2 (GM-CSF) expressing and (C) IL17A express- ing cells (scale bars indicate normalized expression). Three different UMAPs with different cellular granularity showing the respective gene expression of in total cells of the BALF (left), total T cells of blood and BALF (middle), and total T cells in BALF (right) from all patients. (D) Immunofluorescence of CD4+ (green) CCR6+ (red) TRM17 cells in the lungs of a deceased patient with COVID-19 infection [nuclear staining 4′,6-diamidino-2-phenylindol (DAPI), blue] (two additional samples are presented in fig. S10B). (E) Combined immunofluorescence (CCR6) and FISH (IL17A) of lung samples from one patient with COVID-19. (F) Concentrations of the indicated cytokines in the BALF of patients with COVID-19 and bacterial pneumonia.
    Figure Legend Snippet: Fig. 5. Cytokine secretion profile and cellular source of GM-CSF. (A) GM-CSF and IL-17A protein in serum of patients with COVD-19 (n = 8) and healthy controls (n = 7) from Hamburg and of patients with moderate (n = 8) or severe COVID-19 (n = 11) from Halle as indicated. Cell map of (B) CSF2 (GM-CSF) expressing and (C) IL17A express- ing cells (scale bars indicate normalized expression). Three different UMAPs with different cellular granularity showing the respective gene expression of in total cells of the BALF (left), total T cells of blood and BALF (middle), and total T cells in BALF (right) from all patients. (D) Immunofluorescence of CD4+ (green) CCR6+ (red) TRM17 cells in the lungs of a deceased patient with COVID-19 infection [nuclear staining 4′,6-diamidino-2-phenylindol (DAPI), blue] (two additional samples are presented in fig. S10B). (E) Combined immunofluorescence (CCR6) and FISH (IL17A) of lung samples from one patient with COVID-19. (F) Concentrations of the indicated cytokines in the BALF of patients with COVID-19 and bacterial pneumonia.

    Techniques Used: Expressing, Gene Expression, Immunofluorescence, Infection, Staining



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    polyclonal primary goat anti cd4 antibody  (R&D Systems)
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    R&D Systems polyclonal primary goat anti cd4 antibody
    Fig. 2. T cell clonality in pulmonary inflammation. (A) Blood-lung activation map of T cells from blood and BALF of all patients: UMAP dimensionality reduction em- bedding of T cells (left); clone size proportion (clone count divided by number of cells per sample) of T cells (middle), and the cytokine secretion score of T cells (right) from COVID-19 and bacterial pneumonia as indicated. (B) UMAP presentation of T cells from BALF of all patients. Clusters were annotated according to gene expression and epitopes measurement of key markers. TCM, T central memory; TSCM, T stem cell–like memory; lncRNA, long noncoding RNA. (C) Ratio of clonal expansion of bacterial pneumonia versus COVID-19 for the major expanded BALF T cell clusters. (D) Subclustering analysis of clonally expanded <t>CD4+</t> T cells of all patients. Clusters were anno- tated according to gene expression presented in the heatmap. (E) Volcano plot showing differential gene expression between TH17 clusters 1 and 2 of all patients. Genes were considered significant with adjust P < 0.05. Nonsignificant genes are shown in black. (F) Heatmap of selected pathogenic gene markers of TH17 cells of all patients in comparison with other T cell clusters. (G) Clone size proportion of T cells in peripheral blood and BALF of patients with COVID-19 and presentation of high abundant clones (clone size > 5) that are shared between BALF and blood and BAL-specific clones as indicated. (H) CD4 migration and tissue residency score of TH17 cluster1 (TRM17) and 2 (TEM17) from all patients. (I) Possible model of intraclonal diversification of CD4+ T cell subsets (left); distribution of two representative BALF clones from a patient with COVID-19 (patient S1 clone239 and clone218) on the UMAP (middle and right). (J) Bar plot of top expanded BALF clones containing TRM17 cells from patients with COVID-19. COVID-19: n = 8 for BALF and n = 7 for blood; bacterial pneumonia: n = 4 for BALF and n = 4 for blood.
    Polyclonal Primary Goat Anti Cd4 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal primary goat anti cd4 antibody/product/R&D Systems
    Average 94 stars, based on 1 article reviews
    polyclonal primary goat anti cd4 antibody - by Bioz Stars, 2026-05
    94/100 stars
    		  Buy from Supplier

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    Fig. 2. T cell clonality in pulmonary inflammation. (A) Blood-lung activation map of T cells from blood and BALF of all patients: UMAP dimensionality reduction em- bedding of T cells (left); clone size proportion (clone count divided by number of cells per sample) of T cells (middle), and the cytokine secretion score of T cells (right) from COVID-19 and bacterial pneumonia as indicated. (B) UMAP presentation of T cells from BALF of all patients. Clusters were annotated according to gene expression and epitopes measurement of key markers. TCM, T central memory; TSCM, T stem cell–like memory; lncRNA, long noncoding RNA. (C) Ratio of clonal expansion of bacterial pneumonia versus COVID-19 for the major expanded BALF T cell clusters. (D) Subclustering analysis of clonally expanded CD4+ T cells of all patients. Clusters were anno- tated according to gene expression presented in the heatmap. (E) Volcano plot showing differential gene expression between TH17 clusters 1 and 2 of all patients. Genes were considered significant with adjust P < 0.05. Nonsignificant genes are shown in black. (F) Heatmap of selected pathogenic gene markers of TH17 cells of all patients in comparison with other T cell clusters. (G) Clone size proportion of T cells in peripheral blood and BALF of patients with COVID-19 and presentation of high abundant clones (clone size > 5) that are shared between BALF and blood and BAL-specific clones as indicated. (H) CD4 migration and tissue residency score of TH17 cluster1 (TRM17) and 2 (TEM17) from all patients. (I) Possible model of intraclonal diversification of CD4+ T cell subsets (left); distribution of two representative BALF clones from a patient with COVID-19 (patient S1 clone239 and clone218) on the UMAP (middle and right). (J) Bar plot of top expanded BALF clones containing TRM17 cells from patients with COVID-19. COVID-19: n = 8 for BALF and n = 7 for blood; bacterial pneumonia: n = 4 for BALF and n = 4 for blood.

    Journal: Science immunology

    Article Title: Clonal expansion and activation of tissue-resident memory-like Th17 cells expressing GM-CSF in the lungs of severe COVID-19 patients.

    doi: 10.1126/sciimmunol.abf6692

    Figure Lengend Snippet: Fig. 2. T cell clonality in pulmonary inflammation. (A) Blood-lung activation map of T cells from blood and BALF of all patients: UMAP dimensionality reduction em- bedding of T cells (left); clone size proportion (clone count divided by number of cells per sample) of T cells (middle), and the cytokine secretion score of T cells (right) from COVID-19 and bacterial pneumonia as indicated. (B) UMAP presentation of T cells from BALF of all patients. Clusters were annotated according to gene expression and epitopes measurement of key markers. TCM, T central memory; TSCM, T stem cell–like memory; lncRNA, long noncoding RNA. (C) Ratio of clonal expansion of bacterial pneumonia versus COVID-19 for the major expanded BALF T cell clusters. (D) Subclustering analysis of clonally expanded CD4+ T cells of all patients. Clusters were anno- tated according to gene expression presented in the heatmap. (E) Volcano plot showing differential gene expression between TH17 clusters 1 and 2 of all patients. Genes were considered significant with adjust P < 0.05. Nonsignificant genes are shown in black. (F) Heatmap of selected pathogenic gene markers of TH17 cells of all patients in comparison with other T cell clusters. (G) Clone size proportion of T cells in peripheral blood and BALF of patients with COVID-19 and presentation of high abundant clones (clone size > 5) that are shared between BALF and blood and BAL-specific clones as indicated. (H) CD4 migration and tissue residency score of TH17 cluster1 (TRM17) and 2 (TEM17) from all patients. (I) Possible model of intraclonal diversification of CD4+ T cell subsets (left); distribution of two representative BALF clones from a patient with COVID-19 (patient S1 clone239 and clone218) on the UMAP (middle and right). (J) Bar plot of top expanded BALF clones containing TRM17 cells from patients with COVID-19. COVID-19: n = 8 for BALF and n = 7 for blood; bacterial pneumonia: n = 4 for BALF and n = 4 for blood.

    Article Snippet: Immunofluorescence microscopy was performed in 1-m paraffin-embedded sections, after 15-min antigen retrieval with pH 9 antigen retrieval solution (Agilent, Santa Clara, CA, USA) and incubation with polyclonal primary goat anti-CD4 antibody (R&D Systems, Minneapolis, MN, USA, AF-379) and rabbit anti-CCR6 antibody (Abcam, Cambridge, UK, ab140768).

    Techniques: Activation Assay, Gene Expression, Comparison, Clone Assay, Migration

    Fig. 5. Cytokine secretion profile and cellular source of GM-CSF. (A) GM-CSF and IL-17A protein in serum of patients with COVD-19 (n = 8) and healthy controls (n = 7) from Hamburg and of patients with moderate (n = 8) or severe COVID-19 (n = 11) from Halle as indicated. Cell map of (B) CSF2 (GM-CSF) expressing and (C) IL17A express- ing cells (scale bars indicate normalized expression). Three different UMAPs with different cellular granularity showing the respective gene expression of in total cells of the BALF (left), total T cells of blood and BALF (middle), and total T cells in BALF (right) from all patients. (D) Immunofluorescence of CD4+ (green) CCR6+ (red) TRM17 cells in the lungs of a deceased patient with COVID-19 infection [nuclear staining 4′,6-diamidino-2-phenylindol (DAPI), blue] (two additional samples are presented in fig. S10B). (E) Combined immunofluorescence (CCR6) and FISH (IL17A) of lung samples from one patient with COVID-19. (F) Concentrations of the indicated cytokines in the BALF of patients with COVID-19 and bacterial pneumonia.

    Journal: Science immunology

    Article Title: Clonal expansion and activation of tissue-resident memory-like Th17 cells expressing GM-CSF in the lungs of severe COVID-19 patients.

    doi: 10.1126/sciimmunol.abf6692

    Figure Lengend Snippet: Fig. 5. Cytokine secretion profile and cellular source of GM-CSF. (A) GM-CSF and IL-17A protein in serum of patients with COVD-19 (n = 8) and healthy controls (n = 7) from Hamburg and of patients with moderate (n = 8) or severe COVID-19 (n = 11) from Halle as indicated. Cell map of (B) CSF2 (GM-CSF) expressing and (C) IL17A express- ing cells (scale bars indicate normalized expression). Three different UMAPs with different cellular granularity showing the respective gene expression of in total cells of the BALF (left), total T cells of blood and BALF (middle), and total T cells in BALF (right) from all patients. (D) Immunofluorescence of CD4+ (green) CCR6+ (red) TRM17 cells in the lungs of a deceased patient with COVID-19 infection [nuclear staining 4′,6-diamidino-2-phenylindol (DAPI), blue] (two additional samples are presented in fig. S10B). (E) Combined immunofluorescence (CCR6) and FISH (IL17A) of lung samples from one patient with COVID-19. (F) Concentrations of the indicated cytokines in the BALF of patients with COVID-19 and bacterial pneumonia.

    Article Snippet: Immunofluorescence microscopy was performed in 1-m paraffin-embedded sections, after 15-min antigen retrieval with pH 9 antigen retrieval solution (Agilent, Santa Clara, CA, USA) and incubation with polyclonal primary goat anti-CD4 antibody (R&D Systems, Minneapolis, MN, USA, AF-379) and rabbit anti-CCR6 antibody (Abcam, Cambridge, UK, ab140768).

    Techniques: Expressing, Gene Expression, Immunofluorescence, Infection, Staining